Extending the Limits of Single Particle Reconstruction
نویسندگان
چکیده
منابع مشابه
Nasal Reconstruction: Extending the Limits
Reconstructing the 3-dimensional structure of the nose requires the maintenance of its aesthetic form and function. Restoration of the correct dimension, projection, skin quality, symmetrical contour, and function remains problematic. Consequently, modern approaches of nasal reconstruction aim at rebuilding the units rather than just covering the defect. However, revising or redoing a failed or...
متن کاملTesting the Limits of the Single Particle Model in 16
We propose to measure the cross section, Rlt, and Alt for the O(e; ep) reaction with higher precision and to much higher missing momentum and missing energy than in E89-003. We will perform this measurement at the same energy and momentum transfer (Q = 0:8 (GeV/c) and ! = 0:445 GeV). This experiment will take advantage of the Hall A high-precision spectrometers and the self-normalizing waterfal...
متن کاملAvoiding Local Optima in Single Particle Reconstruction
In single-particle reconstruction, a 3D structure is reconstructed from a large number of randomly oriented 2D projections, using techniques related to computed tomography. Unlike in computed tomography, however, the orientations of the projections must be estimated at the same time as the 3D structure, and hence the reconstruction process can be error-prone, converging to an incorrect local op...
متن کاملParticle segmentation algorithm for flexible single particle reconstruction
As single particle cryo-electron microscopy has evolved to a new era of atomic resolution, sample heterogeneity still imposes a major limit to the resolution of many macromolecular complexes, especially those with continuous conformational flexibility. Here, we describe a particle segmentation algorithm towards solving structures of molecules composed of several parts that are relatively flexib...
متن کاملVolumetric restrictions in single particle 3DEM reconstruction
3D electron microsscopy aims at the reconstruction of density volumes corresponding to the electrostatic potential distribution of macro-molecules. There are many factors limiting the resolution achievable when this technique is applied to biological macromolecules: microscope imperfections, molecule flexibility, lack of projections from certain directions, unknown angular distribution, noise, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2009
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927609098225